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Tõnis Laasfeld
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Revisiting the resazurin-based sensing of cellular viability: Widening the application horizon
D Lavogina, H Lust, MJ Tahk, T Laasfeld, H Vellama, N Nasirova, ...
Biosensors 12 (4), 196, 2022
422022
Image-based cell-size estimation for baculovirus quantification
T Laasfeld, S Kopanchuk, A Rinken
Biotechniques 63 (4), 161-168, 2017
182017
The constitutive activity of melanocortin‐4 receptors in cAMP pathway is allosterically modulated by zinc and copper ions
R Link, S Veiksina, MJ Tahk, T Laasfeld, P Paiste, S Kopanchuk, A Rinken
Journal of neurochemistry 153 (3), 346-361, 2020
162020
Binding assay for characterization of protein kinase inhibitors possessing sub-picomolar to sub-millimolar affinity
H Sinijarv, S Wu, T Ivan, T Laasfeld, K Viht, A Uri
Analytical Biochemistry 531, 67-77, 2017
132017
Structure-Based Design of High-Affinity Fluorescent Probes for the Neuropeptide Y Y1 Receptor
C Müller, J Gleixner, MJ Tahk, S Kopanchuk, T Laasfeld, M Weinhart, ...
Journal of Medicinal Chemistry 65 (6), 4832-4853, 2022
122022
Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live‐cell microscopy
A Allikalt, T Laasfeld, M Ilisson, S Kopanchuk, A Rinken
The FEBS Journal 288 (5), 1514-1532, 2021
112021
Budded baculoviruses as a receptor display system to quantify ligand binding with TIRF microscopy
T Laasfeld, R Ehrminger, MJ Tahk, S Veiksina, KR Kolvart, M Min, ...
Nanoscale 13 (4), 2436-2447, 2021
112021
BRET-and fluorescence anisotropy-based assays for real-time monitoring of ligand binding to M2 muscarinic acetylcholine receptors
L Grätz, T Laasfeld, A Allikalt, CG Gruber, A Pegoli, MJ Tahk, ML Tsernant, ...
Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1868 (3), 118930, 2021
92021
Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labeled Ligands Binding to MC4 Receptors …
S Veiksina, MJ Tahk, T Laasfeld, R Link, S Kopanchuk, A Rinken
G protein-coupled receptor screening assays: methods and protocols, 119-136, 2021
82021
Practical segmentation of nuclei in brightfield cell images with neural networks trained on fluorescently labelled samples
D Fishman, SO Salumaa, D Majoral, T Laasfeld, S Peel, J Wildenhain, ...
Journal of Microscopy 284 (1), 12-24, 2021
72021
Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors?
MJ Tahk, J Torp, MAS Ali, D Fishman, L Parts, L Grätz, C Müller, M Keller, ...
Open Biology 12 (6), 220019, 2022
62022
Viability fingerprint of glioblastoma cell lines: Roles of mitotic, proliferative, and epigenetic targets
D Lavogina, T Laasfeld, M Vardja, H Lust, J Jaal
Scientific Reports 11 (1), 20338, 2021
62021
ArtSeg—Artifact segmentation and removal in brightfield cell microscopy images without manual pixel-level annotations
MAS Ali, K Hollo, T Laasfeld, J Torp, MJ Tahk, A Rinken, K Palo, L Parts, ...
Scientific Reports 12 (1), 11404, 2022
42022
Fluorescence based HTS-compatible ligand binding assays for dopamine D3 receptors in baculovirus preparations and live cells
MJ Tahk, T Laasfeld, E Meriste, J Brea, MI Loza, M Majellaro, M Contino, ...
Frontiers in Molecular Biosciences 10, 1119157, 2023
22023
UT-GPCR001 microscopy of ligand binding to M4 muscarinic receptor in live CHO-K1-hM4 cells
MJ Tahk, J Torp, MAS Ali, D Fishman, L Parts, L Grätz, C Müller, M Keller, ...
University of Tartu, Institute of Chemistry, Chair of Bioorganic chemistry, 2022
22022
Exploring the molecular players behind the potentiation of chemotherapy effects by durvalumab in lung adenocarcinoma cell lines
M Saar, J Jaal, A Meltsov, T Laasfeld, H Lust, S Kasvandik, D Lavogina
Pharmaceutics 15 (5), 1485, 2023
12023
ArtSeg: Rapid Artifact Segmentation and Removal in Brightfield Cell Microscopy Images
MAS Ali, K Hollo, T Laasfeld, J Torp, MJ Tahk, A Rinken, K Palo, L Parts, ...
bioRxiv, 2022.01. 24.477467, 2022
12022
cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation
D Lavogina, T Laasfeld, MJ Tahk, O Kukk, A Allikalt, S Kopanchuk, ...
G Protein-Coupled Receptor Screening Assays: Methods and Protocols, 179-192, 2021
12021
Illuminating Neuropeptide Y Y4 Receptor Binding: Fluorescent Cyclic Peptides with Subnanomolar Binding Affinity as Novel Molecular Tools
J Gleixner, S Kopanchuk, L Grätz, MJ Tahk, T Laasfeld, S Veikšina, ...
ACS Pharmacology & Translational Science 7 (4), 1142-1168, 2024
2024
Exploring the Molecular Players behind the Potentiation of Chemotherapy Effects by Durvalumab in Lung Adenocarcinoma Cell Lines. Pharmaceutics 2023, 15, 1485
M Saar, J Jaal, A Meltsov, T Laasfeld, H Lust, S Kasvandik, D Lavogina
2023
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